Targeted Protein Degradation (TPD)
Targeted Protein Degradation (TPD) and other targeted protein degradation strategies offer a novel approach to eliminate disease-driving proteins that may be difficult to address through conventional inhibition. BioDuro integrates degrader design, degradation biology, DMPK, and translational expertise to help advance programs from concept through preclinical evaluation.
By combining chemistry, biology, DMPK, and bioanalytical expertise within one collaborative platform, our teams help reduce handoff challenges and accelerate decision-making throughout Targeted Protein Degradation development.
Target Engagement & Ternary Complexes
Understand degrader activity earlier
Evaluate cell permeability, intracellular target engagement, and ternary complex formation using NanoBRET, TR-FRET, SPR, and complementary assay approaches.
Quantitative Degradation Biology
Quantitatively measure endogenous target degradation
Monitor degradation profiles using HiBiT, NanoLuc, AlphaLISA, JESS, flow cytometry, and kinetic degradation assays.
Integrated Discovery Expertise
Integrated chemistry, biology, and DMPK support
Cross-functional teams help accelerate degrader optimization from early discovery through translational evaluation.
Supporting Every Stage of the Degradation Pathway
Targeted Protein Degradation (TPD) development requires a coordinated understanding of target engagement, degradation biology, and downstream functional effects. Our scientists apply complementary chemistry, biology, and translational approaches to evaluate degrader performance from early mechanistic studies through functional validation.
Cell Permeability & Target Engagement
Evaluate intracellular binding, occupancy, and degrader permeability using NanoBRET, HTRF, SPR, and complementary assay platforms.
Ternary Complex Formation
Characterize interactions between the target protein, degrader, and E3 ligase through kinetic and endpoint assay approaches.
Target Ubiquitination
Assess whether degrader candidates successfully initiate ubiquitination and downstream degradation signaling.
Proteasome Recruitment
Confirm recruitment of degradation machinery using mechanistic assay approaches designed for targeted protein degradation studies.
Target Protein Degradation
Quantitatively monitor endogenous protein degradation using HiBiT, NanoLuc, JESS, Western blot, AlphaLISA, and flow cytometry workflows.
Functional & Translational Biology
Connect degradation activity to downstream biological outcomes including viability, pharmacology, pathway modulation, and phenotypic response.
Key Technologies for Targeted Protein Degradation Evaluation
BioDuro applies multiple orthogonal assay platforms to characterize target engagement, ternary complex formation, and target degradation throughout PROTAC discovery programs.
HiBiT-Based Target Degradation Analysis
Customized HiBiT cell lines enable sensitive, real-time measurement of endogenous target degradation in living cells. Kinetic degradation studies provide quantitative insights into degrader performance and mechanism.
Key outputs include:
- Dmax (maximum degradation)
- DC50 (degradation potency)
- Degradation rate
- Time at Dmax
- Protein recovery
